| General description | Developed to promote the optimal expansion of adult human T cells, Stemline T Cell Expansion Medium demonstrates significantly greater expansion (55%) when compared to alternative media, and viability greater than 95%. Additionally, flow cytometry confirms that with Stemline, a proper CD4/CD8 ratio is maintained. In an ex vivo functional assay (51Chromium Release Assay), T cells expanded in Stemline medium proved to be highly functional and possessed cytolytic potential greater than T cells expanded in serum-containing medium (RPMI with 10% fetal bovine serum). In an in vivo functional assay (GvHD Induction), human T lymphocytes expaned in Stemline medium were injected into NOD/SCIDβ2M mice (n=12). Engraftment, perivascular infiltration, and lethal GvHD were observed by day 15 in 100% of mice, demonstrating excellent in vivo expansion and functionality.
Stemline T Cell Expansion Medium is free of serum and all other animal-derived components with the exceptions of human serum albumin, cholesterol, and transferrin. This exclusion increases performance consistency and elimates safety risks associated with potential adventitious agents.
Produced in a GMP state-of-the-art facility with an available device master file (DMF), Stemline T Cell Expansion Medium is clearly an excellent choice for clinical applications. |
